Fluorescence Spectroscopy to Characterize Protein Aggregates and Particles.

Poole R, Hawe A, Jiskoot W, Braeckmans K.

This chapter contains sections titled:

  • Introduction

  • Introduction to Luminescence

  • Intrinsic Fluorescence

  • Use of Dyes (Extrinsic Fluorescence)

  • Steady‐State Fluorescence

  • Time‐Resolved Fluorescence

  • Fluorescence Quenching

  • Steady‐State and Time‐Resolved Fluorescence Anisotropy

  • Fluorescence Correlation Spectroscopy

  • Single‐Particle Tracking

  • In Vivo Aggregate Characterization

  • Case Studies

  • Summary and Conclusions

  • References

Analysis of Aggregates and Particles in Protein Pharmaceuticals, 1st edition (Mahler HC and Jiskoot W, Eds.)


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