Ion-exchange chromatography (IEX)
Ion-exchange chromatography (IEX) is a widely used, high-throughput analytical technique that allows the separation of analytes based on differences in net surface charge.
The technique does not require organic solvents and is therefore frequently applied to characterize proteins in their native / active form. We offer, IEX on high-performance liquid chromatography (HPLC) instruments coupled with UV-, fluorescence-, refractive index- and/or multi-angle laser light scattering (MALLS) detection. We are experienced in performing IEX on a large selection of columns from various vendors, offering different bead-sizes, matrix materials and functional groups. Mobile phase compositions, particularly salt concentration and pH are optimized method specifically. With many years of experience in developing IEX methods, we are happy to apply our know-how during the development of a tailor-made IEX method for you.
Coriolis is also highly flexible in implementing existing methods from our customers to allow comparability.