Reversed-phase chromatography (RP-HPLC / RP-UPLC)
Reversed-phase chromatography is a commonly used, high-throughput analytical technique that allows the separation of analytes based on differences in hydrophobicity.
We offer, reversed-phase chromatography on high-performance liquid chromatography (HPLC) instruments coupled with UV-, fluorescence-, refractive index- and/or multi-angle laser light scattering (MALLS) detection. Further, ultra-high-performance chromatography (UPLC) instruments with UV detection are available, offering reduced analysis times, requiring lower sample and mobile phase volumes and delivering better resolution. We are experienced in performing reversed-phase chromatography on a large selection of columns from various vendors. With many years of experience in developing reversed-phase chromatography methods, we are happy to apply our know-how during the development of a tailor-made RP method for you. Coriolis is also highly flexible in implementing existing methods from our customers to allow comparability.