Capillary gel electrophoresis (cGE)
Capillary gel electrophoresis (cGE) allows for the qualitative analysis of molecular weight and quantitative analysis of protein aggregates and fragments
cGE is a high-resolution and automated variation of the SDS-PAGE technique. In general, electrophoresis is a separation technique based on the migration of charged molecules in response to an electric field, toward the electrode of opposite charge. It is performed mainly in polyacrylamide gels. In cGE, the gel is located inside a capillary through which the sample components must migrate. The larger the molecular weight, the longer the migration time. A detection system, mostly UV or fluorescence detection, detects and quantifies the migrating species. Molecular weight is determined in reference to a standard.
When compared to conventional SDS-PAGE, cGE allows for a much higher resolution and faster sample analysis and has a lower limit of detection. Moreover, automation makes cGE attractive for use during formulation development, stability studies and forced degradation studies.