Fourier transform infrared (FTIR) microscopy

particle_characterization

Fourier transform infrared (FTIR) microscopy enables visualization and identification of particles in therapeutic protein solutions.


The technique is suitable for particles larger than 20 µm using the reflection–absorption spectroscopy mode: the particles are isolated on a filter, preferably a metal-coated membrane or a gold filter, which allows light that has passed the sample to be reflected by the metal surface and pass through the sample again. Major benefits of FTIR microscopy are the possibility to distinguish between non- proteinaceous and proteinaceous particles and to identify the nature of the non-proteinaceous particles.

FTIR measures the absorption of light due to vibrations of the molecule in the wavelength range from 0.8 to 1000 µm (usually expressed as wavenumbers from 12,500 cm^-1 to 10 cm^-1). The resulting spectra can be used to identify a wide range of compounds by comparing the measured spectra to spectral databases. FTIR microscopy is typically used as a research tool during troubleshooting and root-cause analysis.

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