Protein particle analysis by Flow Cytometry
Coriolis Pharma is a leading contract laboratory providing analytical service of protein aggregation testing including flow cytometry analysis for subvisible particle analysis.
Flow cytometry, which traditionally has been used to characterize cells, finds an increasing application in the field of protein particle analysis in the submicron and micron size range.
In flow cytometry, particles are hydrodynamically focused in a sheath liquid and passed by a laser light source. This method allows simultaneous measurements of light scattering (forward-and side-scattering) and fluorescence from each individual particle which passes through the flow cell. The size range that can be covered is roughly 0.2 to 150 μm. When proteins are fluorescently labeled (mainly using covalent dyes), particle analysis in complex media, such as biological fluids becomes possible.
Also, using flow cytometry, a differentiation between silicone oil and labeled protein particles is feasible, in addition to other methods, e.g. Micro-Flow Imaging (MFI) and Resonant Mass Measurement (RMM, Archimedes).