Nanoparticle Tracking Analysis (NTA, Nanosight)
Coriolis Pharma is specialized in providing contract analytical service of subvisible particle analysis, including Nanoparticle Tracking Analysis (NTA) using Nanosight® instruments.
NTA is a relatively new technique which combines laser light scattering microscopy and a video imaging system for sizing of particles between approximately 50 nm and 1000 nm. After recording a video of the sample illuminated by a laser, the NTA software identifies and tracks the individual particles in the field of view (appearing as point-scatterers) which are moving by Brownian motion. The rate of diffusion (speed) of each individual particle captured on film is related to its size according to a modified Stokes–Einstein relationship.
A syringe pump ensures continuous sample flow and improves the statistical significance especially for low concentrated samples.
Using the total number of measured particles in a given size range, the concentration of particles per size bin can be calculated by extrapolation using an approximate measuring volume estimated by the manufacturer. Detection limits (smallest size) depend on the refractive index of the nanoparticles analyzed.
The NTA method is an interesting research method which accesses a unique size range and is considered a more quantitative alternative to DLS. NTA measures individual particles and therefore provides superior size resolution as compared to DLS, which is a population method.
Nanoparticle tracking analysis is a valuable tool for particle characterization, including, the analysis of viral and other nano-particles, VLP based vaccines, or liposomal products.
Need to know:
Required sample amount ~ 500 -1000 µl per sample (triplicate measurement)